Preparation and Application of Core-shell Chloramphenicol Molecularly Imprinted Polymer Based on MIL-101
以合成的金属有机框架材料MIL-101为基底，氯霉素（CAP）为模板分子，采用溶胶凝胶法，制备核壳型CAP分子印迹聚合物（MIL-101@MIP）。通过X射线衍射光谱（XRD）、傅里叶红外光谱（FTIR）、扫描电镜（SEM）、透射电镜（TEM）对MIL-101与MIL-101@MIP的结构和形貌进行了表征。结果表明，MIL-101为规整八面体结构，在其表面覆盖了有序、多孔的印迹聚合物壳层。测试了分子印迹聚合物的静态、动态和选择性吸附性能。MIL-101@MIP对CAP的最大吸附量为66.56 mg/g，22.5 min达到吸附平衡，且对CAP有较高的特异识别能力。将其作为固相萃取填料，结合HPLC法，对水产品中的CAP进行分析，加标回收率为75.0%~103.7%，相对标准偏差为1.2%~3.9%（n=5）。
Core-shell chloramphenicol (CAP) molecularly imprinted polymer was prepared by sol-gel method with chloramphenicol as template molecule and metal-organic framework material MIL-101 as substrate. The structure and morphology of MIL-101 and molecularly imprinted polymer were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), scanning electron microscope (SEM) and transmission electron microscope (TEM). The results showed that MIL-101 had a regular octahedral structure, and the surface was covered with ordered and porous imprinted polymers. The kinetics and selective adsorption properties of molecularly imprinted polymers were tested. The maximum adsorption capacity of the imprinted polymer was 66.56 mg/g, and the adsorption equilibrium reached within 22.5 min. The imprinted polymer had high selective recognition ability to chloramphenicol. The molecularly imprinted polymers were used as solid phase extraction filler for analysis of chloramphenicol in aquatic products by HPLC method, with recoveries of 75.0%-103.7% and relative standard deviations of 1.2%-3.9% (n=5).